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Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

Anti-IL32 Antibody

HPA029397
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL32
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL32 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9235
  • Target name
    IL32
  • Target description
    interleukin 32
  • Target synonyms
    IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, interleukin-32, interleukin-32 eta, interleukin-32 small, interleukin-32 theta, natural killer cell transcript 4, natural killer cells protein 4, tumor necrosis factor alpha-inducing factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24001
  • Protein Name
    Interleukin-32
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of IL32 using anti-IL32 antibody (A03286). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL32 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL32 antigen affinity purified polyclonal antibody (Catalog # A03286) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL32 at approximately 19KD. The expected band size for IL32 is at 15KD.
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