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Immunohistochemical staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.

Anti-IMMT-25ul

HPA036165
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IMMT-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ICC, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16891
  • Protein Name
    MICOS complex subunit MIC60
  • Scientific Description
    Rabbit Polyclonal Anti-IMMT Antibody against Human inner membrane protein, mitochondrial. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitofilin/IMMT using anti-Mitofilin/IMMT antibody (A04102-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse HEPA1/6 whole cell lysates, Lane 7: mouse MFC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofilin/IMMT antigen affinity purified polyclonal antibody (Catalog # A04102-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofilin/IMMT at approximately 80-90 kDa. The expected band size for Mitofilin/IMMT is at 84 kDa.
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