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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity with granular pattern in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity with granular pattern in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity with granular pattern in glandular cells.

Anti-IMPAD1 Antibody

HPA009411
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBPNT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IMPAD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54928
  • Target name
    BPNT2
  • Target description
    3'(2'), 5'-bisphosphate nucleotidase 2
  • Target synonyms
    GPAPP, IMP 3, IMP-3, IMPA3, IMPAD1, Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase, Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase, IMPase 3, golgi-resident PAP phosphatase, golgi-resident nucleotide phosphatase, inositol monophosphatase domain containing 1, inositol monophosphatase domain-containing protein 1, inositol-1(or 4)-monophosphatase 3, myo-inositol monophosphatase A3, phosphoadenosine phosphate 3'-nucleotidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NX62
  • Protein Name
    Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of BPNT2 using anti-BPNT2 antibody (A11341-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BPNT2 antigen affinity purified polyclonal antibody (Catalog # A11341-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BPNT2 at approximately 42 kDa. The expected band size for BPNT2 is at 39,42 kDa.
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