Anti-influenza [2-12C]

Absolute Antibody

Anti-influenza [2-12C]

9

This antibody is specific for H1N1pdm09 hemagglutinin glycoprotein (HA). Influenza A virus has one of 16 possible hemagglutinin (HA) surface proteins and one of 9 possible neuraminidase (NA) surface proteins. The hemagglutinin protein facilitates viral at

This 2-12C antibody was isolated from a donor that was exposed to A/California/07/2009 virus. It was used as a control antibody alongside with other mAbs in enzyme-linked immunosorbent, indirect immunofluorescent, virus titration, hemagglutination inhibition (HAI), and microneutralization (MN) assays for the assessment of the binding activity of HA head-specific and head cross-reactive antibodies on cells infected with the variant viruses, and for the subsequent mapping of the HA1 K163 residue in former H1N1 viruses (Huang et al., 2015). This 2-12C antibody was reported to select a variant virus with HA substitutions K130E and G170R and, to retain the capacity to neutralize clade 6B viruses at <10 ng/ml similar to the ferret antisera (Huang et al., 2015).

ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

2-12C

Mouse

IgG2a

Hemagglutinin

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203