Anti-Influenza A virus NS1 [NS1-23-1]

Absolute Antibody

Anti-Influenza A virus NS1 [NS1-23-1]

7

To research Akt phosphorylation, western blotting with this antibody was preformed on MDCK and Vero cells infected with A/Puerto Rico/8/34, B/Lee/40 or B/Maryland/59 (Ehrhardt et al, 2007; pmid:17715214). This antibody was used to asses the viral protein expression in A549, HEK293 and Vero cells via Western blot (Kathum et al, 2016; pmid:26687707). To elucidate the ability of NS1 to bind RNA, immunoprecipitation was preformed on cell lysates from A549 cells transfected with wt NS1 or mutated versions of NS1 (Kathum et al, 2016; pmid:26687707). For analysis of NS1-RIG-I complex formation, HEK293 cells were transfected with a pCAGGS-Flag-RIG-I construct. The cell lysate was then co-immunprecipitated using this antibody (Kathum et al, 2016; pmid:26687707). Further, the co-immunoprecipitated NS1 was detected by Western blot using this antibody (Kathum et al, 2016; pmid:26687707).

ImmunoPrecipitation, Western Blot

WB; IP

Research Use Only

Non-structural protein 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203