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Anti-Interferon gamma [XMG1.2], Mouse IgG2a, kappa

AB03914-2.0
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetIfng
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Interferon gamma [XMG1.2], Mouse IgG2a, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original antibody (rat IgG1, kappa) was effective in both ELISA and two blocking bioassays (Cherwinski et al., 1987; PMID: 2960769). This antibody was used successfully in flow cytometry (Sander et al., 1993; PMID: 8288874). This antibody was used in vivo to block and neutralize IFN-gamma (Suzuki and Joh, 1994; PMID: 8202451) (Beatty and Paterson, 2001; PMID: 11160282).
  • Applications
    Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    XMG1.2
  • Gene ID15978
  • Target name
    Ifng
  • Target description
    interferon gamma
  • Target synonyms
    gamma interferon; If; If2f; Ifg; IFN-g; IFN-gamma; interferon 2f; interferon gamma
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP01580
  • Protein Name
    Interferon gamma
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Sandwich ELISA - Recombinant mouse Interferon gamma protein standard curve. Use in combination with reagents from Mouse Interferon gamma ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0375).
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Lane 1: Mouse Raw264.7-PMA Lysates; Lane 2: Mouse Lymph Lysates; Lane 3: Mouse Spleen Lysates; Lane 4: Mouse Thymus Lysates; Lane 5: Rat Lymph Lysates; Lane 6: Rat Thymus Lysates; Lane 7: Human Jurkat-TPA cell Lysates; Lane 8: Human Jurkat cell Lysates; Lane 9: Human HepG2 cell Lysates; Lane 10: Recombinant human IFN gamma Protein (100ng) (bs-0388P). Probed with IFN gamma polyclonal Antibody, unconjugated (bs-0480R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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