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Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Anti-INTS12 Antibody

HPA035772
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetINTS12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-INTS12 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57117
  • Target name
    INTS12
  • Target description
    integrator complex subunit 12
  • Target synonyms
    INT12, PHF22, SBBI22, integrator complex subunit 12, PHD finger protein 22, hypothetical nuclear factor SBBI22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CB8
  • Protein Name
    Integrator complex subunit 12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of INTS12 using anti-INTS12 antibody (A11402-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS12 antigen affinity purified polyclonal antibody (Catalog # A11402-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTS12 at approximately 68 kDa. The expected band size for INTS12 is at 49 kDa.
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