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Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.

Anti-INTS14 Antibody

HPA040651
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetINTS14
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-INTS14
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81556
  • Target name
    INTS14
  • Target description
    integrator complex subunit 14
  • Target synonyms
    C15orf44; integrator complex subunit 14; UPF0464 protein C15orf44; von Willebrand factor A domain containing 9; von Willebrand factor A domain-containing protein 9; VWA9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96SY0
  • Protein Name
    Integrator complex subunit 14
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: INTS14 antibody at 4microg/ml Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 10, 49, 51 kDa Observed band size: 58 kDa
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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts and leydig cells.
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Figure 1. Western blot analysis of INTS14 using anti-INTS14 antibody (A32420). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS14 antigen affinity purified polyclonal antibody (Catalog # A32420) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTS14 at approximately 68 kDa. The expected band size for INTS14 is at 57 kDa.
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