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Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.

Anti-IRF7 Antibody

HPA052757
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIRF7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IRF7
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3665
  • Target name
    IRF7
  • Target description
    interferon regulatory factor 7
  • Target synonyms
    IMD39; interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92985
  • Protein Name
    Interferon regulatory factor 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IRF7 using anti-IRF7 antibody (A00115-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF7 antigen affinity purified polyclonal antibody (Catalog # A00115-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF7 at approximately 54 kDa. The expected band size for IRF7 is at 54 kDa.
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