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Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.

Anti-IRGQ Antibody

HPA050338
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetIRGQ
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IRGQ
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID126298
  • Target name
    IRGQ
  • Target description
    immunity related GTPase Q
  • Target synonyms
    FKSG27; immunity-related GTPase family Q protein; immunity-related GTPase family, Q; immunity-related GTPase family, Q1; IRGQ1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WZA9
  • Protein Name
    Immunity-related GTPase family Q protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IRGQ using anti-IRGQ antibody (A17389-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A2780 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRGQ antigen affinity purified polyclonal antibody (Catalog # A17389-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRGQ at approximately 68 kDa. The expected band size for IRGQ is at 63 kDa.
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