Figure 2. IHC analysis of ISG15/Ucrp using anti-ISG15/Ucrp antibody (PB9951). ISG15/Ucrp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ISG15/Ucrp Antibody (PB9951) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of ISG15/Ucrp using anti-ISG15/Ucrp antibody (PB9951). ISG15/Ucrp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ISG15/Ucrp Antibody (PB9951) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-ISG15 Antibody Picoband(r)
PB9951-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetIsg15
Overview
- SupplierBoster Bio
- Product NameAnti-ISG15 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID100038882
- Target nameIsg15
- Target descriptionISG15 ubiquitin-like modifier
- Target synonymsG1p2, IGI15, IP17, Irfp, UCRP, ubiquitin-like protein ISG15, interferon, alpha-inducible protein, interferon-induced 15 kDa protein, interferon-induced 15-KDa protein, interferon-induced 17 kDa protein, interferon-stimulated protein (15 kDa), interferon-stimulated protein 15, ubiquitin cross-reactive protein
- HostRabbit
- IsotypeIgG
- Protein IDQ64339
- Protein NameUbiquitin-like protein ISG15
- Scientific DescriptionBoster Bio Anti-ISG15 Antibody Picoband® catalog # PB9951. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetIsg15
- SizePrice