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Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli, while cells in tubules are negative.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli, while cells in tubules are negative.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli, while cells in tubules are negative.

Anti-ITGA8 Antibody

HPA003432
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITGA8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ITGA8 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8516
  • Target name
    ITGA8
  • Target description
    integrin subunit alpha 8
  • Target synonyms
    integrin alpha-8, integrin, alpha 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53708
  • Protein Name
    Integrin alpha-8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ITGA8 using anti-ITGA8 antibody (A06636-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA8 antigen affinity purified polyclonal antibody (Catalog # A06636-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA8 at approximately 180 kDa. The expected band size for ITGA8 is at 117 kDa.
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