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Immunohistochemical staining of human colon shows moderate nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate nuclear and cytoplasmic positivity in glandular cells.

Anti-ITGB3BP Antibody

HPA028463
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITGB3BP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ITGB3BP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23421
  • Target name
    ITGB3BP
  • Target description
    integrin subunit beta 3 binding protein
  • Target synonyms
    CENP-R, CENPR, HSU37139, NRIF3, TAP20, centromere protein R, beta 3 endonexin, beta3-endonexin, integrin beta 3 binding protein (beta3-endonexin), integrin beta-3-binding protein, nuclear receptor-interacting factor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13352
  • Protein Name
    Centromere protein R
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Centromere protein R/ITGB3BP using anti-Centromere protein R/ITGB3BP antibody (A09098-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Centromere protein R/ITGB3BP antigen affinity purified polyclonal antibody (Catalog # A09098-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Centromere protein R/ITGB3BP at approximately 20 kDa. The expected band size for Centromere protein R/ITGB3BP is at 20,23 kDa.
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