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ProductsBack/Anti-ITGB4-25ul
Immunohistochemical staining of human urinary bladder shows moderate membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate membranous positivity in urothelial cells.

Anti-ITGB4-25ul

HPA036349
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ITGB4-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16144
  • Protein Name
    Integrin beta-4
  • Scientific Description
    Rabbit Polyclonal Anti-ITGB4 Antibody against Human integrin, beta 4. Validated for Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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MSDS

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Figure 1. Western blot analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (A01015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat ovary tissue lysates, Lane 8: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 4/ITGB4 antigen affinity purified polyclonal antibody (Catalog # A01015-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 4/ITGB4 at approximately 210KD. The expected band size for Integrin beta 4/ITGB4 is at 202KD.
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