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Immunohistochemical staining of human Heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Immunohistochemical staining of human Heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.

Anti-ITPK1 Antibody

HPA055230
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITPK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ITPK1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3705
  • Target name
    ITPK1
  • Target description
    inositol-tetrakisphosphate 1-kinase
  • Target synonyms
    ITRPK1, inositol-tetrakisphosphate 1-kinase, inositol 1,3,4-trisphosphate 5/6-kinase, ins(1,3,4)P(3) 5/6-kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13572
  • Protein Name
    Inositol-tetrakisphosphate 1-kinase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ITPK1 using anti-ITPK1 antibody (A09106-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPK1 antigen affinity purified polyclonal antibody (Catalog # A09106-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPK1 at approximately 42 kDa. The expected band size for ITPK1 is at 46 kDa.
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Atlas Antibodies
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Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 10% SDS PAGE GTX107974 diluted at 1:1000
Product group Antibodies
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