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Immunohistochemical staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.

Anti-ITPR1 Antibody

HPA014765
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITPR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ITPR1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3708
  • Target name
    ITPR1
  • Target description
    inositol 1,4,5-trisphosphate receptor type 1
  • Target synonyms
    ACV, CLA4, INSP3R1, IP3R, IP3R1, PPP1R94, SCA15, SCA16, SCA29, inositol 1,4,5-trisphosphate-gated calcium channel ITPR1, IP3 receptor, IP3R 1, inositol 1,4,5 trisphosphate receptor, inositol 1,4,5-triphosphate receptor, type 1, protein phosphatase 1, regulatory subunit 94, type 1 InsP3 receptor, type 1 inositol 1,4,5-trisphosphate receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14643
  • Protein Name
    Inositol 1,4,5-trisphosphate receptor type 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP3 receptor antigen affinity purified polyclonal antibody (Catalog # PB9225) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP3 receptor at approximately 290 kDa. The expected band size for IP3 receptor is at 314 kDa.
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