Anti-JAML [HL4E10 ]

Absolute Antibody

Anti-JAML [HL4E10 ]

7

HL4E10 was found to be specific for JAML, as this protein was identified as the ligand for the mAb from immunoprecipitation (Witherden et al, 2010). Biochemical binding studies showed that HL4E10 bound the JAML D2 Ig domain, which was corroborated with a

HL4E10 has been used to detect JAML by both IP and FC (Witherden et al, 2010). IF labelling showed that JAML colocalises with the T cell receptor.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Other Application

IP; FC; IF; FACS; crystallisation; SPR

Research Use Only

Monoclonal

HL4E10

JAML

junction adhesion molecule like

adhesion molecule AMICA; adhesion molecule interacting with CXADR antigen 1; adhesion molecule, interacts with CXADR antigen 1; AMICA; AMICA1; CREA7-1; CREA7-4; dendritic-cell specific protein CREA7-1; dendritic-cell specific protein CREA7-4; Gm638; junctional adhesion molecule-like

Rabbit

IgG

Junctional adhesion molecule-like

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

An Armenian hamster was immunised with the 7-17 dendritic epidermal T cell line. Splenocytes were obtained from immunised hamsters and fused with the X63Ag8.653 myeloma cell line to generate a hybridoma.

-20°C,2°C to 8°C

12352203