Anti-Jo-1 [5B5]

Absolute Antibody

Anti-Jo-1 [5B5]

7

The antibody is specific for Jo-1. Histidyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, it catalyzes the ATP-dependent ligation of histidine to the 3-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediat

The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected Jo-1 by western blot analysis (Hof et al., 2008; PMID: 18062792).

Western Blot, ELISA

ELISA; WB

Research Use Only

Monoclonal

5B5

HARS1

histidyl-tRNA synthetase 1

CMT2W; HARS; HisRS; histidine translase; histidine--tRNA ligase, cytoplasmic; HRS; Jo-1 antigen; USH3B

Rabbit

IgG

Histidine--tRNA ligase, cytoplasmic

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.

-20°C,2°C to 8°C

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