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Immunohistochemical staining of human thyroid gland shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate nuclear positivity in glandular cells.

Anti-JUN Antibody

AMAB91587
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetJUN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-JUN Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL9662
  • Conjugate
    Unconjugated
  • Gene ID3725
  • Target name
    JUN
  • Target description
    Jun proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, AP1, c-Jun, cJUN, p39, transcription factor Jun, Jun activation domain binding protein, activator protein 1, enhancer-binding protein AP1, jun oncogene, proto-oncogene c-Jun, proto-oncogene cJun, transcription factor AP-1, transcription factor AP-1 subunit Jun, v-jun avian sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05412
  • Protein Name
    Transcription factor Jun
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (A02038-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody (Catalog # A02038-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Jun/JUN at approximately 43 kDa. The expected band size for c-Jun/JUN is at 43 kDa.
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Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-c-Jun Polyclonal Antibody (bs-0670R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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