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ProductsBack/Anti-JUND-25ul
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Anti-JUND-25ul

HPA063029
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-JUND-25ul
  • Delivery Days Customer
    6
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ICC, IHC, ChIP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.05
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17535
  • Protein Name
    Transcription factor jun-D
  • Scientific Description
    Rabbit Polyclonal Anti-JUND Antibody against Human jun D proto-oncogene. Validated for Chromatin Immunoprecipitation, Immunofluorescence and Immunohistochemistry
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JunD using anti-JunD antibody (A05609-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U2OS whole cell lysates, Lane 8: human U251 whole cell lysates, Lane 9: rat lung tissue lysates, Lane 10: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JunD antigen affinity purified polyclonal antibody (Catalog # A05609-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JunD at approximately 38 kDa, 42 kDa. The expected band size for JunD is at 35 kDa.
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