Anti-kappa light chain [NH3/41.34]

Absolute Antibody

Anti-kappa light chain [NH3/41.34]

7

This antibody recognises human immunoglobulin kappa light chain. The antibody does not cross react with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4.

NH3/41.34 antibody detects kappa light chains from a human IgGs and Bence-Jones protein samples via Western blot under reducing conditions (Thorpe et al., 1984). This antibody is equally able to detect full IgGs via Western blot when the samples are transferred from the non-reducing SDS polyacrylamide gels (Thorpe et al., 1984). Further, this antibody was capable of detecting IgGs via Western blot from native gels run under acidic conditions (Thorne et al., 1984). Finally, the specificity of the antibody for human IgG1 was confirmed by ELISA analysis. No cross reactivity with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 was detected.

Western Blot

WB

Research Use Only

Monoclonal

NH3/41.34

Mouse

IgG1

Human

Human

This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.

-20°C,2°C to 8°C

12352203