Bio-Connect

Anti-kappa light chain [NH3/41.34]

Research Use Only
Ab00855-1.1
Absolute Antibody
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-kappa light chain [NH3/41.34]
  • Delivery Days Customer
    7
  • Antibody Specificity
    This antibody recognises human immunoglobulin kappa light chain. The antibody does not cross react with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4.
  • Application Supplier Note
    NH3/41.34 antibody detects kappa light chains from a human IgGs and Bence-Jones protein samples via Western blot under reducing conditions (Thorpe et al., 1984). This antibody is equally able to detect full IgGs via Western blot when the samples are transferred from the non-reducing SDS polyacrylamide gels (Thorpe et al., 1984). Further, this antibody was capable of detecting IgGs via Western blot from native gels run under acidic conditions (Thorne et al., 1984). Finally, the specificity of the antibody for human IgG1 was confirmed by ELISA analysis. No cross reactivity with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 was detected.
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    NH3/41.34
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203