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Anti-KCNH2 Antibody

144-02968
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKCNH2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-KCNH2 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3757
  • Target name
    KCNH2
  • Target description
    potassium voltage-gated channel subfamily H member 2
  • Target synonyms
    ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1, voltage-gated inwardly rectifying potassium channel KCNH2, eag homolog, eag-related protein 1, ether-a-go-go-related gene potassium channel 1, ether-a-go-go-related potassium channel protein, ether-a-go-go-related protein 1, human ether-a-go-go-related, long QT syndrome type 2, potassium channel, voltage gated eag related subfamily H, member 2, potassium voltage-gated channel, subfamily H (eag-related), member 2, voltage-gated potassium channel subunit Kv11.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12809
  • Protein Name
    Voltage-gated inwardly rectifying potassium channel KCNH2
  • Scientific Description
    KCNH2 Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KCNH2 using anti-KCNH2 antibody (A00781-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates Lane 2: human U2OS whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNH2 at approximately 150KD. The expected band size for KCNH2 is at 127KD.
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