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Anti-KDM6B [RAB-C330]

Ab01801-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation
Product group Antibodies
ReactivityHuman
TargetKDM6B
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-KDM6B [RAB-C330]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: IP-MS; IF.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation
  • Applications Supplier
    IP-MS; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C330
  • Gene ID23135
  • Target name
    KDM6B
  • Target description
    lysine demethylase 6B
  • Target synonyms
    JMJD3, NEDCFSA, NEDSST, lysine-specific demethylase 6B, [histone H3]-trimethyl-L-lysine(27) demethylase 6B, jmjC domain-containing protein 3, jumonji domain containing 3, histone lysine demethylase, jumonji domain-containing protein 3, lysine (K)-specific demethylase 6B
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO15054
  • Protein Name
    Lysine-specific demethylase 6B
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing KDM6B protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody (A01309-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM6B antigen affinity purified polyclonal antibody (Catalog # A01309-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM6B at approximately 177KD. The expected band size for KDM6B is at 177KD.
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