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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Anti-KIF15 Antibody

HPA035517
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKIF15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KIF15 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56992
  • Target name
    KIF15
  • Target description
    kinesin family member 15
  • Target synonyms
    BRDCS2, HKLP2, KLP2, KNSL7, NY-BR-62, kinesin-like protein KIF15, kinesin-like 7, kinesin-like protein 2, kinesin-like protein 7, serologically defined breast cancer antigen NY-BR-62
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NS87
  • Protein Name
    Kinesin-like protein KIF15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of KIF15 using anti-KIF15 antibody (A05983-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF15 antigen affinity purified polyclonal antibody (Catalog # A05983-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF15 at approximately 150-160KD. The expected band size for KIF15 is at 150-160KD.
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