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Immunohistochemical staining of human testis shows nuclear positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows nuclear positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows nuclear positivity in cells of seminiferus ducts.

Anti-KIF2A-25ul

HPA004716
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KIF2A-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00139
  • Protein Name
    Kinesin-like protein KIF2A
  • Scientific Description
    Rabbit Polyclonal Anti-KIF2A Antibody against Human kinesin heavy chain member 2A. Validated for Immunohistochemistry and Western Blot
  • Reactivity
    Human, Rat
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIF2A using anti-KIF2A antibody (A03979-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF2A antigen affinity purified polyclonal antibody (Catalog # A03979-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF2A at approximately 110 kDa. The expected band size for KIF2A is at 110 kDa.
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