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Immunofluorescence staining of mouse red nucleus shows immunoreactivity in neuronal cell bodies.
Immunofluorescence staining of mouse red nucleus shows immunoreactivity in neuronal cell bodies.
Immunofluorescence staining of mouse red nucleus shows immunoreactivity in neuronal cell bodies.

Anti-KIF5A Antibody

HPA004469
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKIF5A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-KIF5A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3798
  • Target name
    KIF5A
  • Target description
    kinesin family member 5A
  • Target synonyms
    ALS25, D12S1889, MY050, NEIMY, NKHC, SPG10, kinesin heavy chain isoform 5A, KIF5A variant protein, kinesin heavy chain neuron-specific 1, kinesin, heavy chain, neuron-specific, neuron-specific kinesin heavy chain, neuronal kinesin heavy chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12840
  • Protein Name
    Kinesin heavy chain isoform 5A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of KIF5A using anti-KIF5A antibody (A03635-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF5A antigen affinity purified polyclonal antibody (Catalog # A03635-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF5A at approximately 117 kDa. The expected band size for KIF5A is at 117 kDa.
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