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Anti-KLK2 Antibody

Anti-KLK2 Antibody

A38782
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-KLK2 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3.3 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to KLK2
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (A03879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human COLO-320 cell lysate, Lane 3: human SK-OV-3 cell lysate, Lane 4: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 2 antigen affinity purified polyclonal antibody (Catalog # A03879) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 2 at approximately 21KD. The expected band size for Kallikrein 2 is at 28KD.
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Human tissue extract (5 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Kallikrein 2 antibody [N3C3] (GTX100526) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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