Anti-KPNA3 Antibody (A84000 | Biotin) (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.