Anti-L-Lactyllysine Rabbit pAb (BSA and Azide Free)

PTM BIO

Anti-L-Lactyllysine Rabbit pAb (BSA and Azide Free)

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Anti-L-Lactyllysine Rabbit pAb (BSA and Azide Free) detects proteins post-translationally modified by L-lactylation on lysine residues. This pan antibody recognizes L-lactylated lysine independent of its surrounding sequences.

Western Blot, ImmunoHistoChemistry Paraffin

WB, IHC-P

Antibody

Research Use Only

Polyclonal

Unconjugated

Rabbit

IgG

Lactate, previously recognized solely as an energy source and metabolic byproduct, has now emerged as a crucial player in cancer biology, especially within the scope of the Warburg effect and its intricate interplay with various cellular processes, including angiogenesis, hypoxia, polarization of macrophages, and T cell activation. Recent investigations have unveiled an unprecedented function of lactate in the realm of histone modification, specifically through lysine L-lactylation, which exerts regulatory control over gene expression. The extent and dynamics of this modification are highly reliant on lactate levels within the cellular microenvironment and can be modulated through the introduction of extracellular lactate in cultured cells or the stimulation of intracellular glycolysis. The acetyltransferase p300 is responsible for introducing lysine L-lactylation, while Class I histone deacetylases (HDAC 1-3) have been identified as an eraser of the lactylation marks on histones. This BSA-, glycerol-, and azide-free antibody is particularly suitable for applications such as mass cytometry.

Stable for 12 months from date of receipt/reconstitution.

All Species

All

Protein A and immunogen affinity purified

Before reconstitution, centrifuge the vial at 12,000 x g for 20 seconds. Add 100 uL sterile, deionized water to achieve a final antibody concentration of 1 mg/ml. After reconstitution, the antibody will be in a 1X PBS solution. Aliquot and store at -80°C. Avoid freeze/thaw cycles.

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