Anti-La Crosse virus, G1 envelope glycoprotein [807.15]

Absolute Antibody

Anti-La Crosse virus, G1 envelope glycoprotein [807.15]

9

This antibody is specific for the Bunyavirus La Crosse G1 viral glycoprotein. According to the original publication (Gonzalez-Scarano et al, 1982), this antibody clone 807.15 was validated to belong to Group A antibodies, which were were virus specific an

In the original publication, this antibody, together with other 22 clones against the the G1 and N proteins of LaCrosse and Tahyna, was characterised by the ELISA assays, immunoprecipitation, neutralisation tests, and hemagglutination inhibition tests (Gonzalez-Scarano et al, 1982). It was used for antigenic taxonomy of Califmia serogroup viruses and for the identification of the California serogroup viruses of North America (Gonzalez-Scarano et al, 1982). It was also used, together with the other anti-La Crosse mAb clones 807.35 and 807.27, in the neutralisation assays of murine leukemia virus pseudotypes of La Crosse and Hantaan Bunyaviruses to validate a system for analysis of cell tropism (Ma et al, 1999). Furthermore, a single immunisation with this antibody was reported to result in a robust immune response and protection against La Crosse virus (Pekosz et al, 1995). Recently, this antibody has been reported in various FACS analyses, for example, to demonstrate that mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide (Plassmeyer et al, 2007), and to suggest that the fusion peptide of La Crosse virus Gc is a determinant of properties associated with neurotoxicity (Soldan et al, 2010).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

807.15

Mouse

IgG1

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203