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ProductsBack/Anti-Lactoferrin [4C5]

Anti-Lactoferrin [4C5]

Ab02772-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetLTF
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Lactoferrin [4C5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This original IgG1 mouse antibody is specific towards human lactoferrin, which was shown in- and outside the cell using immunofluorescence (Knapp et al., 1993; PMID: 8210650). One group used the original mouse IgG1 antibody with flow cytochemistry to identify the immunophenotype of several rhesus monkeys (Weisgrau et al., 2019; PMID: 30395351).
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Applications Supplier
    FC; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4C5
  • Gene ID4057
  • Target name
    LTF
  • Target description
    lactotransferrin
  • Target synonyms
    epididymis luminal protein 110; GIG12; growth-inhibiting protein 12; HEL110; HLF2; kaliocin-1; lactoferricin; lactoferroxin; lactotransferrin; LF; neutrophil lactoferrin; talalactoferrin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02788
  • Protein Name
    Lactotransferrin
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original mouse IgG1 version of this antibody was raised by immunizing mice against human lactoferrin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lactoferrin using anti-Lactoferrin antibody (A00633-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lactoferrin antigen affinity purified polyclonal antibody (Catalog # A00633-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lactoferrin at approximately 85 kDa. The expected band size for Lactoferrin is at 78 kDa.
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