Anti-lambda light chain [6-H5]

Absolute Antibody

Anti-lambda light chain [6-H5]

9

This antibody is specific for the lambda light chain of ferret B cell immunoglobulin.

This antibody is specific for the ferret lambda light chain of B cell surface receptor (BCR) and enables segreation of kappa and lambda B cells representing great improvement over polyclonal anti-ferret Ig. It can be used as an indicator of B cell malignancies such as multiple myeloma. It was screened for reactivity against ferret peripheral blood mononuclear cells by FCM what demonstrated specificity for CD79B+ B cells (Kirchenbaum and Ross, 2017; pmid:28286781). It is suitable for Western Blot, ELISA and Flow Cytometry applications. Enzyme-linked immunosorbent assay with the murine version of this antibody (ELISA) was used to assess the reactivity against purified ferret Ig and to determine the IgG subclass and concentration of the respective mAb (Kirchenbaum and Ross, 2017; pmid:28286781). Further, a competitive ELISA was performed using unlabeled and biotinylated MalphaF-Ig mAb to identify overlapping epitope recognition (Kirchenbaum and Ross, 2017; pmid:28286781). To characterize the specificity of the murine version of this antibody a western blot on ferret immunoglobin was preformed (Kirchenbaum and Ross, 2017; pmid:28286781).

Flow Cytometry, Western Blot, ELISA

Research Use Only

Monoclonal

6-H5

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Ferret

-20°C,2°C to 8°C

12352203