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Immunohistochemical staining of human Testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-LARP4B Antibody

HPA036566
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLARP4B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LARP4B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23185
  • Target name
    LARP4B
  • Target description
    La ribonucleoprotein 4B
  • Target synonyms
    KIAA0217; La ribonucleoprotein domain family member 4B; La ribonucleoprotein domain family, member 5; la-related protein 4B; la-related protein 5; LARP5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92615
  • Protein Name
    La-related protein 4B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LARP4B using anti-LARP4B antibody (A08433-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARP4B antigen affinity purified polyclonal antibody (Catalog # A08433-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARP4B at approximately 95 kDa. The expected band size for LARP4B is at 81 kDa.
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Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.
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