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Immunofluorescent staining of human cell line RH-30 shows localization to nuclear membrane.
Immunofluorescent staining of human cell line RH-30 shows localization to nuclear membrane.
Immunofluorescent staining of human cell line RH-30 shows localization to nuclear membrane.

Anti-LBR Antibody

HPA062236
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetLBR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LBR Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3930
  • Target name
    LBR
  • Target description
    lamin B receptor
  • Target synonyms
    C14SR, DHCR14B, LMN2R, PHA, PHASK, TDRD18, delta(14)-sterol reductase LBR, 3-beta-hydroxysterol Delta (14)-reductase, C-14 sterol reductase, delta-14-SR, integral nuclear envelope inner membrane protein, sterol C14-reductase, tudor domain containing 18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14739
  • Protein Name
    Delta(14)-sterol reductase LBR
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LBR using anti-LBR antibody (A01238-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human HL-60 whole cell lysates Lane 3: human K562 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: human HEK293 whole cell lysates Lane 6: human U2OS whole cell lysates Lane 7: human U-937 whole cell lysates Lane 8: human Caco-2 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (Catalog # A01238-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBR at approximately 65-71KD. The expected band size for LBR is at 71KD.
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