Anti-LDL receptor [12G4]

Absolute Antibody

Anti-LDL receptor [12G4]

7

The binding specificity of this antibody was detected using ELISA. This antibody is reported to be used to identify LDL-R expression level in cancer cell lines like HepG2, HeLa, MCF-7, Jurkat, Ramos, HuH7, A549, MDA-MB-231 and Hek293 using flow cytometry. It was found that HepG2 and HeLa cells express LDL-R most strongly. This antibody is also reported to cross react with mouse, rat and hamster LDL receptors. It strongly cross reacts with C2C12, CHO-K1, and YB2/0 cells. 12G4 antibody binding site is the same as that of LDLs and it competes with LDLs for binding site on A549 cells. This antibody was also used for the immunohistochemical analysis of LDL-receptors on cells from the colon, testes, pancreas, gastric mucosa, the basal cell layer of the buccal and exocervical mucosa, and the various renal tubules. It was also used for immunohistochemical analysis of mammary adenocarcinomas (US20090175789).

Flow Cytometry, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

ELISA; FC; IHC; block

Research Use Only

Low-density lipoprotein receptor

-20°C,2°C to 8°C

12352203