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Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.

Anti-LEMD3 Antibody

HPA025078
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLEMD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LEMD3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23592
  • Target name
    LEMD3
  • Target description
    LEM domain containing 3
  • Target synonyms
    MAN1, inner nuclear membrane protein Man1, LEM domain-containing protein 3, LEMD3/MKX fusion, MAN antigen 1, integral inner nuclear membrane protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2U8
  • Protein Name
    Inner nuclear membrane protein Man1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAN1/LEMD3 using anti-MAN1/LEMD3 antibody (A04957-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAN1/LEMD3 antigen affinity purified polyclonal antibody (Catalog # A04957-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAN1/LEMD3 at approximately 120 kDa. The expected band size for MAN1/LEMD3 is at 100 kDa.
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