Figure 1. IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350). Leptin Receptor/LEPR was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Leptin Receptor/LEPR Antibody (A00350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350). Leptin Receptor/LEPR was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Leptin Receptor/LEPR Antibody (A00350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Leptin Receptor/LEPR Antibody
A00350-CARRIER-FREE
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetLEPR
Overview
- SupplierBoster Bio
- Product NameAnti-Leptin Receptor/LEPR Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID3953
- Target nameLEPR
- Target descriptionleptin receptor
- Target synonymsCD295; huB219; LEP-R; LEPRD; leptin receptor; OB receptor; OBR; OB-R
- HostRabbit
- IsotypeIgG
- Protein IDP48357
- Protein NameLeptin receptor
- Scientific DescriptionBoster Bio Anti-Leptin Receptor/LEPR Antibody Picoband® catalog # A00350. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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