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Anti-LGALS2 (Center) Antibody

102-27591
RayBiotech
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLGALS2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-LGALS2 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB22637
  • Gene ID3957
  • Target name
    LGALS2
  • Target description
    galectin 2
  • Target synonyms
    beta-galactoside-binding lectin L-14-II; gal-2; galectin-2; HL14; lactose-binding lectin 2; lectin, galactoside-binding, soluble, 2; S-Lac lectin 2
  • Host
    Rabbit
  • Protein IDP05162
  • Protein Name
    Galectin-2
  • Scientific Description
    Rabbit Anti-LGALS2 (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galectin 2/LGALS2 using anti-Galectin 2/LGALS2 antibody (A04114-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 2/LGALS2 antigen affinity purified polyclonal antibody (Catalog # A04114-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 2/LGALS2 at approximately 15KD. The expected band size for Galectin 2/LGALS2 is at 15KD.
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