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Immunohistochemical staining of human skin shows positivity in epidermal cells.
Immunohistochemical staining of human skin shows positivity in epidermal cells.
Immunohistochemical staining of human skin shows positivity in epidermal cells.

Anti-LGALS7 Antibody

HPA001549
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLGALS7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LGALS7
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3963
  • Target name
    LGALS7
  • Target description
    galectin 7
  • Target synonyms
    GAL7; galectin-7; lectin, galactoside-binding, soluble, 7; LGALS7A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP47929
  • Protein Name
    Galectin-7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galectin-7/LGALS7 using anti-Galectin-7/LGALS7 antibody (A12693-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skin tissue lysates, Lane 2: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-7/LGALS7 antigen affinity purified polyclonal antibody (Catalog # A12693-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin-7/LGALS7 at approximately 15 kDa. The expected band size for Galectin-7/LGALS7 is at 15 kDa.
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