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Immunohistochemical staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.

Anti-LIMK2 Antibody

HPA008183
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLIMK2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LIMK2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3985
  • Target name
    LIMK2
  • Target description
    LIM domain kinase 2
  • Target synonyms
    LIM domain kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53671
  • Protein Name
    LIM domain kinase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIM kinase 2 using anti-LIM kinase 2 antibody (PB9717). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Brain Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: Mouse Thymus Tissue Lysate at 50ug, Lane 4: Mouse Testis Tissue Lysate at 50ug, Lane 5: 293T Whole Cell Lysate at 40ug, Lane 6: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIM kinase 2 antigen affinity purified polyclonal antibody (Catalog # PB9717) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIM kinase 2 at approximately 72 kDa. The expected band size for LIM kinase 2 is at 72 kDa.
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