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Immunohistochemical staining of human thyroid gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong granular cytoplasmic positivity in glandular cells.

Anti-LIN7C Antibody

HPA051118
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetLIN7C
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LIN7C
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55327
  • Target name
    LIN7C
  • Target description
    lin-7 homolog C, crumbs cell polarity complex component
  • Target synonyms
    LIN-7 protein 3; LIN-7C; LIN-7-C; MALS3; MALS-3; mammalian lin-seven protein 3; protein lin-7 homolog C; VELI3; veli-3; vertebrate lin-7 homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NUP9
  • Protein Name
    Protein lin-7 homolog C
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIN7C using anti-LIN7C antibody (A07929-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN7C antigen affinity purified polyclonal antibody (Catalog # A07929-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN7C at approximately 22 kDa. The expected band size for LIN7C is at 22 kDa.
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