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Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Anti-LINGO2 Antibody

HPA016633
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLINGO2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LINGO2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID158038
  • Target name
    LINGO2
  • Target description
    leucine rich repeat and Ig domain containing 2
  • Target synonyms
    LERN3, LRRN6C, leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2, leucine rich repeat neuronal 6C, leucine-rich repeat neuronal protein 3, leucine-rich repeat neuronal protein 6C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L985
  • Protein Name
    Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of LINGO2 using anti-LINGO2 antibody (A12658-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LINGO2 antigen affinity purified polyclonal antibody (Catalog # A12658-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LINGO2 at approximately 68 kDa. The expected band size for LINGO2 is at 68 kDa.
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