Anti-Lipid A [S1-15]

Absolute Antibody

Anti-Lipid A [S1-15]

9

The antibody recognizes the carbohydrate backbone of lipid A. The antibody binds to the 4-monophosphoryl GlcN disaccharide. The antibody does not cross react with lipopolysaccharides (LPS). The antibody cross reacts with ssDNA. Lipid A is the lipid compon

In passive immunohemolysis, the antibody reacted with bisphosphoryl compounds as well as with the 4-monophosphoryl partial structures (Kuhn et al., 1992; PMID 1375194). The specificity of the antibody was determined by ELISA analysis. The crystal structures of the Fab fragment in complex with lipid A carbohydrate backbone and in the unliganded form were determined by X-ray diffraction, showing the binding mechanism of the antibody is a lock and key type. Further, the structure of the Fab fragment in complex with single-stranded DNA fragment 5(dT) oligonucleotide was determined. The antibody bound two separate oligonucleotides by their terminal 5- and 3-phosphate groups (Haji-Ghassemi et al., 2015; PMID: 26085093). Solid-phase enzyme immunoassay shown the antibody binds better to the glycoconjugate at low antigen concentrations and better to the lipid A when high concentrations of antigen were used (Brade et al., 1993). The antibody was tested against the synthetic lipid A precursor Ia and compound B1047, a precursor Ia, by enzyme immunoassay. The antibody bound to precursor Ia, but not to compound B 1047 or Escherichia coli Re LPS (Brade et al., 1997; PMID: 9284181).

ELISA, Other Application

Research Use Only

Monoclonal

S1-15

Mouse

IgG2b

Bacteria

-20°C,2°C to 8°C

12352203