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Immunohistochemical staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.
Immunohistochemical staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.
Immunohistochemical staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.

Anti-LMNB1 Antibody

AMAB91251
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetLMNB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LMNB1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3929
  • Conjugate
    Unconjugated
  • Gene ID4001
  • Target name
    LMNB1
  • Target description
    lamin B1
  • Target synonyms
    ADLD; lamin-B1; LMN; LMN2; LMNB; MCPH26
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20700
  • Protein Name
    Lamin-B1
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (Catalog # PB9611) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 72 kDa. The expected band size for Lamin B1 is at 66 kDa.
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