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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-LMNTD1 Antibody

HPA045911
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLMNTD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LMNTD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID160492
  • Target name
    LMNTD1
  • Target description
    lamin tail domain containing 1
  • Target synonyms
    IFLTD1, LMNARS1, PAS1C1, lamin tail domain-containing protein 1, intermediate filament tail domain containing 1, intermediate filament tail domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N9Z9
  • Protein Name
    Lamin tail domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IFTLD1/LMNTD1 using anti-IFTLD1/LMNTD1 antibody (A16307-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFTLD1/LMNTD1 antigen affinity purified polyclonal antibody (Catalog # A16307-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFTLD1/LMNTD1 at approximately 43 kDa. The expected band size for IFTLD1/LMNTD1 is at 43 kDa.
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