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Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Anti-LNPEP Antibody

HPA043642
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetLNPEP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-LNPEP Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4012
  • Target name
    LNPEP
  • Target description
    leucyl and cystinyl aminopeptidase
  • Target synonyms
    CAP, IRAP, P-LAP, PLAP, leucyl-cystinyl aminopeptidase, AT (4) receptor, angiotensin IV receptor, cystinyl aminopeptidase, insulin-regulated aminopeptidase, insulin-regulated membrane aminopeptidase, insulin-responsive aminopeptidase, otase, oxytocinase, placental leucine aminopeptidase, vasopressinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIQ6
  • Protein Name
    Leucyl-cystinyl aminopeptidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of LNPEP using anti-LNPEP antibody (A05092-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LNPEP antigen affinity purified polyclonal antibody (Catalog # A05092-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LNPEP at approximately 117 kDa. The expected band size for LNPEP is at 117 kDa.
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