Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (A04921). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # A04921) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD.
Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (A04921). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # A04921) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD.
Anti-LOC134147/CMBL Antibody Picoband(r)
A04921
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetCmbl
Overview
- SupplierBoster Bio
- Product NameAnti-LOC134147/CMBL Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID69574
- Target nameCmbl
- Target descriptioncarboxymethylenebutenolidase homolog
- Target synonyms2310016A09Rik, carboxymethylenebutenolidase homolog, carboxymethylenebutenolidase-like (Pseudomonas)
- HostRabbit
- IsotypeIgG
- Scientific DescriptionBoster Bio Anti-LOC134147/CMBL Antibody Picoband® catalog # A04921. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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Product group Antibodies
Anti-LOC134147/CMBL Antibody Picoband(r)A04921-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
TargetCmbl
- SizePrice