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Anti-LOX 1/Olr1 Antibody

ORB2607717
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetOlr1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-LOX 1/Olr1 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.25-0.5microg/ml, Mouse, Rat ELISA, 0.1-0.5microg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    RPE
  • Gene ID108078
  • Target name
    Olr1
  • Target description
    oxidized low density lipoprotein (lectin-like) receptor 1
  • Target synonyms
    LOX-1, SR-EI, Scare1, oxidized low-density lipoprotein receptor 1, lectin-like oxLDL receptor 1, lectin-like oxidized LDL receptor 1, lectin-type oxidized LDL receptor 1, ox-LDL receptor 1, oxidised low density lipoprotein (lectin-like) receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9EQ09
  • Protein Name
    Oxidized low-density lipoprotein receptor 1
  • Scientific Description
    Anti-LOX 1/Olr1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Olr1 using anti-Olr1 antibody (A00760-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse testicular tissue lysates, Lane 6: mouse smooth muscle tissue lysates, Lane 7: mouse SP20 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Olr1 antigen affinity purified polyclonal antibody (Catalog # A00760-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Olr1 at approximately 52KD. The expected band size for Olr1 is at 31KD.
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ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetOlr1
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