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Figure 1. Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human prostatic carcinoma tissue (PCT) lysates, Lane 2: human cervical carcinoma tissue (CCAT) lysates, Lane 3: human gallbladder carcinoma tissue (GBCT) lysates, Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa.
Figure 1. Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human prostatic carcinoma tissue (PCT) lysates, Lane 2: human cervical carcinoma tissue (CCAT) lysates, Lane 3: human gallbladder carcinoma tissue (GBCT) lysates, Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa.
Figure 1. Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human prostatic carcinoma tissue (PCT) lysates, Lane 2: human cervical carcinoma tissue (CCAT) lysates, Lane 3: human gallbladder carcinoma tissue (GBCT) lysates, Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa.

Anti-LOX 1/OLR1 Antibody Picoband(r)

PA1832-APC
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetOLR1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-LOX 1/OLR1 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID4973
  • Target name
    OLR1
  • Target description
    oxidized low density lipoprotein receptor 1
  • Target synonyms
    CLEC8A; C-type lectin domain family 8 member A; hLOX-1; lectin-type oxidized LDL receptor 1; LOX1; LOXIN; ox LDL receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; SCARE1; scavenger receptor class E, member 1; SLOX1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78380
  • Protein Name
    Oxidized low-density lipoprotein receptor 1
  • Scientific Description
    Boster Bio Anti-LOX 1/OLR1 Antibody catalog # PA1832. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203