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Anti-LRCH4 (Center) Antibody

102-25171
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetLRCH4
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-LRCH4 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB56329
  • Gene ID4034
  • Target name
    LRCH4
  • Target description
    leucine rich repeats and calponin homology domain containing 4
  • Target synonyms
    LRN, LRRN1, LRRN4, PP14183, leucine-rich repeat and calponin homology domain-containing protein 4, leucine rich repeat neuronal 4, leucine-rich neuronal protein, leucine-rich repeats and calponin homology (CH) domain containing 4
  • Host
    Rabbit
  • Protein IDO75427
  • Protein Name
    Leucine-rich repeat and calponin homology domain-containing protein 4
  • Scientific Description
    Rabbit Anti-LRCH4 (Center) Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRCH4 using anti-LRCH4 antibody (A13633-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRCH4 antigen affinity purified polyclonal antibody (Catalog # A13633-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRCH4 at approximately 84 kDa. The expected band size for LRCH4 is at 73 kDa.
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